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KMID : 1177720150090010045
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Journal of Alternatives to Animal Experiments
2015 Volume.9 No. 1 p.45 ~ p.52
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Integrated Approaches to Assess Skin Sensitizing Potential of Substances
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Kim Ji-Hoon
Cho Sun-A
Lee John-Hwan
An Su-Sun
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Abstract
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Skin sensitization is a key parameter for risk assessments of cosmetic ingredient. Recent regulation changes for safety and ethical issues against animal testing call for the development of animal-free alternative tests for evaluating skin sensitizing potential of chemicals. The current available test methods, which have been validated by ECVAM, show good relevance to animal test. However, a single alternative method is not able to assess skin sensitization due to the complexity of the steps involved in this endpoint. To cover the complexity and reflect mechanistic understanding of sequential processes within biological system, the adverse outcome pathway (AOP) conceptual framework, new strategy through integrating data from reliable in vitro test methods have been investigated. In this study, the common chemical set composed of 25 sensitizers and 14 non-sensitizers was tested by three reliable in vitro assays, chemical reactivity assay (Spectro-DPRA), Nrf-2 based assay (ARE assay), and Dendritic cell based assay (h-CLAT) addressing the processes of development of skin sensitization. The predictive performance of each or combinations of assays were compared. Based on these data, two different integrated approaches were designed and individual performances were analyzed for the hazard identification of sensitizers.
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KEYWORD
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Skin sensitization, Integrated Testing Strategy, ITS, Adverse Outcome Pathway, AOP
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